Aedes aegypti Liverpool gene set annotation

Genes were annotated by merging Ensembl and TIGR prediction sets. The Ensembl automatic analysis pipeline used either a GeneWise/Exonerate model from a known protein or a set of aligned cDNAs/ESTs followed by an ORF prediction. GeneWise/Exonerate models are further combined with available aligned cDNAs/ESTs to annotate UTRs (for more information see V.Curwen et al., Genome Res. 2004 14:942-50). TIGR annotations were based on Augustus (M. Stanke), GlimmerHMM (M. Pertea), Genie (D. Kulp), and Twinscan (M. Brent) gene predictions, coupled with protein and EST genome alignments.